Other
Part:BBa_K2789023:Design
Designed by: Tianyi Chang Group: iGEM18_WHU-China (2018-10-06)
PPX+RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 872
Design Notes
It is a coding sequence plus a RBS. Thus you can just add another coding sequence to make two proteins expressed together.
Source
It is from E.coli.But we didn't succeed to have a sample by PCR from E.coli.BL21. This suggested that it came from another strain of E.coli.