Other

Part:BBa_K2789023:Design

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)


PPX+RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 872


Design Notes

It is a coding sequence plus a RBS. Thus you can just add another coding sequence to make two proteins expressed together.


Source

It is from E.coli.But we didn't succeed to have a sample by PCR from E.coli.BL21. This suggested that it came from another strain of E.coli.


References